Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells

Oleksiievets, Nazar; Sargsyan, Yelena; Christoph Thiele, Jan; Mougios, Nikolaos; Sograte‐Idrissi, Shama; Nevskyi, Oleksii; Gregor, Ingo; Opazo, Felipe; Thoms, Sven; Enderlein, Jörg; Tsukanov, Roman

doi:10.1038/s42003-021-02976-4

Public

Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells

Published in Communications Biology • Jan 11, 2022

Authors:

Nazar Oleksiievets

Yelena Sargsyan

Jan Christoph Thiele

Abstract

DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we comb...

Subject

Fluorescence

Fluorescence-lifetime imaging microscopy

DNA

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