Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Aggarwal, Abhi; Liu, Rui; Chen, Yang; J Ralowicz, Amelia; J Bergerson, Samuel; Tomáška, Filip; Hanson, Timothy; Hasseman, J.; Reep, Daniel; Tsegaye, Getahun; Yao, Pantong; Ji, Xiang; Kloos, Marinus; Walpita, Deepika; Patel, Ronak; Mohr, Manuel; W Tilberg, Paul; Mohar, Boaz; GENIE Project Team, The; L. Looger, Loren; S. Marvin, Jonathan; B. Hoppa, Michael; Konnerth, Arthur; Kleinfeld, David; R. Schreiter, Eric; Podgorski, Kaspar

doi:10.1101/2022.02.13.480251

Public

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Published in bioRxiv (Cold Spring Harbor Laboratory) • Feb 15, 2022

Authors:

Abhi Aggarwal

Rui Liu

Yang Chen

Abstract

Abstract The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit saturating activation kinetics and are excluded from post-synaptic densities, limiting their ability to distinguish synaptic ...

Subject

Neurotransmission

Glutamate receptor

Kinetics

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