Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples

Papadakis, George; Pantazis, Alexandros; Fikas, Nikolaos; Chatziioannidou, S.; Tsiakalou, V.; Michaelidou, Kleita; Pogka, Vasiliki; Megariti, Maria; Vardaki, M.; Giarentis, K.; Heaney, Judith; Nastouli, Eleni; Karamitros, Timokratis; A. Mentis, Alexios‐Fotios; Zafiropoulos, Alexandros; Sourvinos, George; Agelaki, Sofia; Gizeli, Electra

doi:10.1038/s41598-022-06632-7

Public

Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples

Published in Scientific Reports • Mar 8, 2022

Authors:

George Papadakis

Alexandros Pantazis

Nikolaos Fikas

Abstract

Loop-mediated isothermal amplification is known for its high sensitivity, specificity and tolerance to inhibiting-substances. In this work, we developed a device for performing real-time colorimetric LAMP combining the accuracy of lab-based quantitative analysis with the simplicity of point-of-care ...

Subject

Loop-mediated isothermal amplification

Detection limit

Computer science

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