Engineered Cas9 extracellular vesicles as a novel gene editing tool

Osteikoetxea, Xabier; Silva, Andreia; Lázaro‐Ibáñez, Elisa; Salmond, Nikki; Shatnyeva, Olga; Stein, Josia; Schick, Jan; Wren, Stephen; Lindgren, Julia; Firth, Mike; Madsen, Alexandra; M. Mayr, Lorenz; Overman, R.; Davies, Rick; Dekker, Niek

doi:10.1002/jev2.12225

Public

Engineered Cas9 extracellular vesicles as a novel gene editing tool

Published in Journal of Extracellular Vesicles • May 1, 2022

Authors:

Xabier Osteikoetxea

Andreia Silva

Elisa Lázaro‐Ibáñez

Abstract

Extracellular vesicles (EVs) have shown promise as biological delivery vehicles, but therapeutic applications require efficient cargo loading. Here, we developed new methods for CRISPR/Cas9 loading into EVs through reversible heterodimerization of Cas9-fusions with EV sorting partners. Cas9-loaded E...

Subject

CRISPR

Cas9

Genome editing

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