Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis

Oleksiievets, Nazar; Mathew, Christeena; Christoph Thiele, Jan; Ignacio Gallea, José; Nevskyi, Oleksii; Gregor, Ingo; Weber, André; Tsukanov, Roman; Enderlein, Jörg

doi:10.1021/acs.nanolett.2c01586

Public

Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis

Published in Nano Letters • Jul 6, 2022

Authors:

Nazar Oleksiievets

Christeena Mathew

Jan Christoph Thiele

Abstract

A recent addition to the toolbox of super-resolution microscopy methods is fluorescence-lifetime single-molecule localization microscopy (FL-SMLM). The synergy of SMLM and fluorescence-lifetime imaging microscopy (FLIM) combines superior image resolution with lifetime information and can be realized...

Subject

Microscopy

Confocal

Optics

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