Fast and visual detection of nucleic acids using a one-step RPA-CRISPR detection (ORCD) system unrestricted by the PAM

Lin, Kangfeng; Guo, Jianguang; Guo, Xiangju; Li, Qinghan; Li, Xiao; Sun, Zhen; Zhao, Zehang; Weng, Jiao; Wu, Jinzhun; Zhang, Rui; Li, Boan

doi:10.1016/j.aca.2023.340938

Public

Fast and visual detection of nucleic acids using a one-step RPA-CRISPR detection (ORCD) system unrestricted by the PAM

Published in Analytica Chimica Acta • Feb 3, 2023

Authors:

Kangfeng Lin

Jianguang Guo

Xiangju Guo

Abstract

CRISPR-Cas12a (Cpf1) is widely used for pathogen detection. However, most Cas12a nucleic acid detection methods are limited by a PAM sequence requirement. Moreover, preamplification and Cas12a cleavage are separate. Here, we developed a one-step RPA-CRISPR detection (ORCD) system unrestricted by the...

Subject

Nucleic acid

Chemistry

CRISPR

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