Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

Agam, Ganesh; Gebhardt, Christian; Popara, Milana; Mächtel, Rebecca; Folz, Julian; Ambrose, Benjamin; Chamachi, Neharika; Chung, Sangyoon; D. Craggs, Timothy; de Boer, Marijn; Grohmann, Dina; Ha, Taekjip; Hartmann, Andreas; Hendrix, Jelle; Hirschfeld, Verena; G. Hübner, Christian; Hugel, Thorsten; Kammerer, Dominik; Kang, Hyun-Seo; N. Kapanidis, Achillefs; Krainer, Georg; Kramm, Kevin; A. Lemke, Edward; Lerner, Eitan; Margeat, Emmanuel; Martens, Kirsten; Michaelis, Jens; Mitra, Jaba; G. Moya Muñoz, Gabriel; B. Quast, Robert; C. Robb, Nicole; Sattler, Michael; Schlierf, Michael; Schneider, Jonathan; Schröder, Tim; Sefer, Anna; Siong Tan, Piau; Thurn, Johann; Tinnefeld, Philip; van Noort, John; Weiss, Shimon; Wendler, Nicolas; Zijlstra, Niels; Barth, Anders; A. M. Seidel, Claus; C. Lamb, Don; Cordes, Thorben

doi:10.1038/s41592-023-01807-0

Public

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

Published in Nature Methods • Mar 27, 2023

Authors:

Ganesh Agam

Christian Gebhardt

Milana Popara

Abstract

Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study of biomolecular structure and dynamics in vitro and in vivo. We performed an international blind study involving 19 laboratories to assess the uncertainty of FRET experiments for proteins with respect to the measu...

Subject

Förster resonance energy transfer

Single-molecule FRET

Biological system

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