Single-cell m6A mapping in vivo using picoMeRIP–seq

Li, Yanjiao; Wang, Yunhao; Vera-Rodríguez, María; C. Lindeman, Leif; Ellevog Skuggen, Linda; M. K. Rasmussen, Erik; Jermstad, Ingunn; Khan, S.; Fosslie, Madeleine; Skuland, Trine; Indahl, Marie; Khodeer, Sherif; Kristine Klemsdal, Eva; Jin, Kang-Xuan; Tomas Dalen, Knut; Fedorcsák, Péter; D. Greggains, Gareth; Lerdrup, Mads; Klungland, Arne; Fai Au, Kin; Arne Dahl, John

doi:10.1038/s41587-023-01831-7

Public

Single-cell m6A mapping in vivo using picoMeRIP–seq

Published in Nature Biotechnology • Jun 22, 2023

Authors:

Yanjiao Li

Yunhao Wang

María Vera-Rodríguez

Abstract

Abstract Current N 6 -methyladenosine (m 6 A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m 6 A RNA immunoprecipitation and sequencing (picoMeRIP–seq) for studying m 6 A in vivo i...

Subject

In vivo

Computational biology

Cell

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