Cytosolic‐enhanced dark Epac‐based <scp>FRET</scp> sensors allow for intracellular <scp>cAMP</scp> detection in live cells via <scp>FLIM</scp>

Zanetti, Giulia; Klarenbeek, Jeffrey; Jalink, Kees

doi:10.1002/1873-3468.15093

Public

Cytosolic‐enhanced dark Epac‐based FRET sensors allow for intracellular cAMP detection in live cells via FLIM

Published in FEBS Letters • Dec 31, 2024

Authors:

Giulia Zanetti

Jeffrey Klarenbeek

Kees Jalink

Abstract

Fluorescence resonance energy transfer (FRET)-based biosensors are powerful tools for studying second messengers with high temporal and spatial resolution. FRET is commonly detected by ratio imaging, but fluorescence lifetime imaging microscopy (FLIM), which measures the donor fluorophore's lifetime...

Subject

Cytosol

Chemistry

Intracellular

Research Assistant

AI chat, annotations, notes & similar papers

Finding related papers...

Discussions

(0)

No comments yet

Be the first to share your thoughts!