North Carolina Sea Grant

Phthalate diesters are chemicals to which humans are ubiquitously exposed. Exposure to certain phthalates during sexual differentiation causes reproductive tract malformations in male rats. In the fetal rat, exposure to the phthalates benzylbutyl phthalate (BBP), di(n)butyl phthalate (DBP), and diethylhexyl phthalate (DEHP) decreases testicular testosterone production and insulin-like 3 hormone mRNA levels. We characterized the dose-response effects of six individual phthalates (BBP, DBP, DEHP, diethyl phthalate [DEP], diisobutyl phthalate [DiBP], and dipentyl phthalate [DPP]) on gestation day (GD) 18 testicular testosterone production following exposure of Sprague-Dawley rats on GD 8-18. BBP, DBP, DEHP, and DiBP were equipotent (ED50 of 440 +/- 16 mg/kg/day), DPP was about threefold more potent (ED50 = 130 mg/kg/day) and DEP had no effect on fetal testosterone production. We hypothesized that coadministration of these five antiandrogenic phthalates would reduce testosterone production in a dose-additive fashion because they act via a common mode of toxicity. In a second study, dams were dosed at 100, 80, 60, 40, 20, 10, 5, or 0% of the mixture. The top dose contained 1300 mg of total phthalates/kg/day including BBP, DBP, DEHP, DiBP (300 mg/kg/day per chemical), and DPP (100 mg DPP/kg/day). This mixture ratio was selected such that each phthalate would contribute equally to the reduction in testosterone. As hypothesized, testosterone production was reduced in a dose-additive manner. Several of the individual phthalates and the mixture also induced fetal mortality, due to pregnancy loss. These data demonstrate that individual phthalates with a similar mechanism of action can elicit cumulative, dose additive effects on fetal testosterone production and pregnancy when administered as a mixture.

Field and laboratory studies were conducted to investigate the mechanisms of weed suppression by cover crops. High-performance liquid chromatograph analysis and a seed germination bioassay demonstrated that rye ( Secale cereale L.) can be leached of its allelochemicals, redried, and used as an inert control for separating physical suppression from other types of interference. In a field study, rye, crimson clover ( Trifolium incarnatum L.), hairy vetch ( Vicia villosa Roth.), barley ( Hordeum vulgare L . ), and a mixture of the four species suppressed the emergence of eastern black nightshade ( Solanum ptycanthum Dun.). Crimson clover inhibited the emergence of eastern black nightshade beyond what could be attributed to physical suppression alone. The emergence of yellow foxtail [ Setaria glauca (L.) Beauv.] was inhibited by rye and barley but not by the other cover crops or the cover crop mixture.

Abstract Growth in fish and other vertebrates is under endocrine control, particularly through the growth hormone (GH)–insulin-like growth factor (IGF) axis. For this reason, it has been of interest to aquaculture researchers and the industry to establish endocrine biomarkers that can both reflect and predict growth rates in fish subject to various biotic and abiotic manipulations. Ultimately, by understanding the hormones that control growth and utilizing them as biomarkers, we hope to achieve optimal growth conditions in the aquaculture environment with less need for lengthy and costly grow-out trials. While the most appropriate endocrine biomarkers for growth can be both species and situation specific, IGF-I may be the most promising candidate for measuring instantaneous growth in fish. This is based on the direct contributions of IGF-I in regulating cell proliferation and ultimately somatic growth, along with its previously established correlations with the specific growth rate in fish under various conditions that alter growth. However, other endocrine indices, such as GH and IGF-binding proteins (IGFBPs), are also important contributors and may in some instances prove a strong corollary to growth rate. This review discusses the potential utility of GH, IGF-I, and IGFBPs as growth biomarkers for those manipulations most relevant to the aquaculture industry, namely, feeding regimen, diet composition, temperature, photoperiod, and stress.

Many rural communities are examining nature tourism options, such as birdwatching (birding), as an economic development strategy. Unfortunately,public media stories, tourism professionals, and biologists often describe birders as a homogeneous group of serious, dedicated, and even fanatical visitors willing to spend large sums of money in their pursuit.Consistent with past research, we contend that birders constitute a hetero-geneous group of recreationists. Using recreational specialization as a conceptual framework, we identified four groups of visitors to a popular birding festival and show that the groups also differ in terms of level of behavioral involvement and setting preferences. Findings from this study will aid community leaders and event organizers in their efforts to target programs, amenities, and promotional materials to distinct segments of the birdwatching population.

ABSTRACT: Hydraulic geometry relationships, or regional curves, relate bankfull stream channel dimensions to watershed drainage area. Hydraulic geometry relationships for streams throughout North Carolina vary with hydrology, soils, and extent of development within a watershed. An urban curve that is the focus of this study shows the bankfull features of streams in urban and suburban watersheds throughout the North Carolina Piedmont. Seventeen streams were surveyed in watersheds that had greater than 10 percent impervious cover. The watersheds had been developed long enough for the streams to redevelop bankfull features, and they had no major impoundments. The drainage areas for the streams ranged from 0.4 to 110.3 square kilometers. Cross‐sectional and longitudinal surveys were conducted to determine the channel dimension, pattern, and profile of each stream and power functions were fitted to the data. Comparisons were made with regional curves developed previously for the rural Piedmont, and enlargement ratios were produced. These enlargement ratios indicated a substantial increase in the hydraulic geometry for the urban streams in comparison to the rural streams. A comparison of flood frequency indicates a slight decrease in the bankfull discharge return interval for the gaged urban streams as compared to the gaged rural streams. The study data were collected by North Carolina State University (NCSU), the University of North Carolina at Charlotte (UNC), and Charlotte Storm Water Services. Urban regional curves are useful tools for applying natural channel design in developed watersheds. They do not, however, replace the need for field calibration and verification of bankfull stream channel dimensions.

Risk-based management approaches to climate adaptation depend on the assessment of potential threats, and their causes, vulnerabilities, and impacts. The refinement of these approaches relies heavily on detailed local knowledge of places and priorities, such as infrastructure, governance structures, and socio-economic conditions, as well as scientific understanding of climate projections and trends. Developing processes that integrate local and scientific knowledge will enhance the value of risk-based management approaches, facilitate group learning and planning processes, and support the capacity of communities to prepare for change. This study uses the Vulnerability, Consequences, and Adaptation Planning Scenarios (VCAPS) process, a form of analytic-deliberative dialogue, and the conceptual frameworks of hazard management and climate vulnerability, to integrate scientific and local knowledge. We worked with local government staff in an urbanized barrier island community (Sullivan’s Island, South Carolina) to consider climate risks, impacts, and adaptation challenges associated with sea level rise and wastewater and stormwater management. The findings discuss how the process increases understanding of town officials’ views of risks and climate change impacts to barrier islands, the management actions being considered to address of the multiple impacts of concern, and the local tradeoffs and challenges in adaptation planning. We also comment on group learning and specific adaptation tasks, strategies, and needs identified.

Compensatory growth (CG) is a period of growth acceleration that exceeds normal rates after animals are alleviated of certain growth-stunting conditions. In hybrid striped bass (HSB, Morone chrysops X Morone saxatilis), 3 weeks of complete feed restriction results in a catabolic state that, when relieved, renders a subsequent phase of CG. The catabolic state was characterized by depressed levels of hepatic Type I and II GH receptor (ghr1, ghr2) and igf1 mRNA, along with considerable decreases in plasma Igf1. The state of catabolism also resulted in significant declines in hepatic igf2 mRNA and in circulating 40 kDa Igf-binding protein (Igfbp). Skeletal muscle expression of ghr2 mRNA was significantly increased. Upon realimentation, specific growth rates (SGRs) were significantly higher than sized-matched controls, indicating a period of CG. Hepatic ghr1, ghr2, igf1 and igf2 mRNA levels along with plasma Igf1 and 40 kDa Igfbp increased rapidly during realimentation. Plasma Igf1 and total hepatic igf2 mRNA were significantly correlated to SGR throughout the study. Skeletal muscle igf1 mRNA also increased tenfold during CG. These data suggest that endocrine and paracrine/autocrine components of the GH-Igf axis, namely igf1, igf2, and ghr1 and ghr2, may be involved in CG responses in HSB, with several of the gene expression variables exceeding normal levels during CG. We also demonstrate that normalization of hepatic mRNA as a function of total liver production, rather than as a fraction of total RNA, may be a more biologically appropriate method of quantifying hepatic gene expression when using real-time PCR.

Four tomato production systems were compared at Columbus and Fremont, Ohio: 1) a conventional system; 2) an integrated system [a fall-planted cover-crop mixture of hairy vetch ( Vicia villosa Roth.), rye ( Secale cereale L.), crimson clover ( Trifolium incarnatum L.), and barley ( Hordeum vulgare L.) killed before tomato planting and left as mulch, and reduced chemical inputs]; 3) an organic system (with cover-crop mixture and no synthetic chemical inputs); and (4) a no-input system (with cover-crop mixture and no additional management or inputs). Nitrogen in the cover-crop mixture above-ground biomass was 220 kg·ha -1 in Columbus and 360 kg·ha -1 in Fremont. Mulch systems (with cover-crop mixture on the bed surface) had higher soil moisture levels and reduced soil maximum temperatures relative to the conventional system. Overall, the cover-crop mulch suppressed weeds as well as herbicide plots, and no additional weed control was needed during the season. There were no differences in the frequency of scouted insect pests or diseases among the treatments. The number of tomato fruit and flower clusters for the conventional system was higher early in the season. In Fremont, the plants in the conventional system had accumulated more dry matter 5 weeks after transplanting. Yield of red fruit was similar for all systems at Columbus, but the conventional system yielded higher than the other three systems in Fremont. In Columbus, there were no differences in economic return above variable costs among systems. In Fremont, the conventional systems had the highest return above variable costs.

Abstract Four separate studies were done on Southern flounder Paralichthys lethostigma larvae during first feeding and metamorphosis to determine the effects of stocking density, salinity, and light intensity on growth and survival. One study used stocking densities of 10, 20, 40, and 80 fish/L during first feeding; the second study compared the growth and survival of larvae stocked at 20 and 33 ppt; and a third experiment evaluated stocking densities of 1/L and 3/L under two different light intensities (1,600 lux vs 340 lux) during metamorphosis. The fourth experiment tested the effects of different salinities (0, 10, 20 and 30 ppt) on larval growth and survival during metamorphosis. Growth and survival (overall 6.9%) were not significantly different ( P > 0.05) for stocking rates up to 80/L. Larvae placed into 20 ppt salinity had survival through first feeding similar to that of larvae raised at 33 ppt. During metamorphosis, light intensity had no effect ( P > 0.05) on growth or survival, but fish stocked at 3/L had significantly lower ( P < 0.05) survival than fish at 1/L. Complete mortality of larvae occurred at 0 ppt. Growth and survival past metamorphosis were not significantly different ( P > 0.05) at 10, 20 and 30 ppt, but unmetamorphosed fish did not survive to day 60 at 10 ppt. Based on these results, practical larviculture of Southern flounder may require a two‐step process with high stocking rates (80 fish/L) through first feeding and lower densities (1/L) through metamorphosis. Fingerling production in fertilized nursery ponds might he possible at salinity as low as 20 ppt.

The Bolivar Peninsula in Texas was severely impacted by Hurricane Ike with strong winds, large waves, widespread inundation, and severe damage. This paper examines the wave and surge climate on Bolivar during the storm and the consequent survival and destruction of buildings. Emphasis is placed on differences between buildings that survived (with varying degrees of damage) and buildings that were completely destroyed. Building elevations are found to be the primary indicator of survival for areas with large waves. Here, buildings that were sufficiently elevated above waves and surge suffered relatively little structural damage, while houses at lower elevations were impacted by large waves and generally completely destroyed. In many areas, the transition from destruction to survival was over a very small elevation range of around 0.5 m. In areas where waves were smaller, survival was possible at much lower elevations. Higher houses that were not inundated still survived, but well-built houses at lower elevations could also survive as the waves were not large enough to cause structural damage. However, the transition height where waves became damaging could not be determined from this study.

Coastal community water infrastructure is increasingly vulnerable to climate-sensitive coastal hazards. Tides, storm surges, rainfall, and salt intrusion affect infrastructure and human health. In case studies of Charleston, South Carolina, and Morehead City, North Carolina, USA, this project sought to advance risk assessment of urban water and wastewater infrastructure and identify linkages to human health impacts as risk evolves with sea level rise. The methodology integrates community infrastructure, health care, emergency resources, geospatial simulation, and a tabletop exercise with planners, emergency managers, public utilities, and health care providers. Resilience is assessed by community participants using interactive online maps, susceptibility indices, and a resilience matrix. Results highlight differential vulnerability, population susceptibility, and elevation uncertainty. We observe similar trends of increasing magnitude, frequency, and impact of flood events on water infrastructure and public health as sea level rises. Implications for tackling challenges across sectors are highlighted for improving coastal resilience.

The cardiovascular and neuromuscular interactions of verapamil and dantrolene were evaluated in 20 chloralose-anesthetized swine. The animals were randomly divided into three groups. Group I, ten animals, received a bolus intravenous injection of 0.1 mg . kg-1 of verapamil followed by the continuous infusion of 5 micrograms . kg-1 . min-1. This group was then randomly divided into two equal subgroups. Five of these animals, Group Ia, continued to receive the verapamil infusion alone. The other five animals, Group Ib, received dantrolene in incremental doses of 1.0, 3.3, and 5.6 mg . kg-1 while the verapamil infusion was continued. An additional group of five animals, Group II, received the same incremental doses of dantrolene but did not receive verapamil. Five control animals, Group III, received the alpha-chloralose anesthetic without dantrolene or verapamil. Neuromuscular function, as measured by twitch height, was affected only by dantrolene, which produced a dose-dependent depression. Verapamil resulted in initial decreases in heart rate, arterial blood pressure, cardiac output, left ventricular dP/dt, and an increase in PR interval. Dantrolene alone produced a mild increase in arterial blood pressure. Dantrolene administration to verapamil-pretreated animals resulted in a profound depression in cardiac function, marked elevation in serum K+ (8.0 +/- 0.7 mEq . l-1), and no change in arterial pH (7.39 +/- 0.02). Cardiac arrest preceded by complete atrioventricular heart block occurred in one animal before and in four animals after the final dantrolene dose was given to animals pretreated with verapamil. Although we cannot extrapolate data from our porcine model to humans, further studies are indicated to help evaluate a possible fatal drug interaction before verapamil and dantrolene are used concomitantly in a clinical setting.

Significant life experience research suggests that the presence of role models, time outdoors, and nature-related media foster pro-environmental behavior, but most research is qualitative. Based on a random sample of middle school students in North Carolina, USA, we found limited positive associations between presence of a role model and time outdoors with behavior and a negative association between watching nature television and environmental knowledge. The strongest predictors of environmental knowledge and behavior were student/teacher ratio and county income levels, respectively. We also found that Native Americans engaged in environmental behaviors more than Caucasians, and that African American and Hispanic students had lower levels of environmental knowledge. Accordingly, life experiences appear less important than promoting small class sizes and addressing challenges associated with lower incomes in schools.

Posthurricane Ike surveys for almost 2,000 individual wood-framed houses were combined with high resolution numerical hindcasts to investigate the collapse limit state arising from overland waves and surge. For this storm, freeboard above wave crests, wave height, current velocity, and construction date were found to be the most important factors determining survival. Regressions show considerable skill in predicting collapse fragilities and will prove useful in assessing vulnerability. The relatively small range of wind speeds available in the data set did not allow accurate assessments of wind effects on collapse. Several ASCE and FEMA force estimates showed relatively low skill in modeling fragility, in large part because their breaking wave assumption was not accurate over much of the range. However, the use of best-estimate wave heights in force modeling greatly improved accuracy of fragility estimates. Despite the positive results of this study, numerous questions remain. It proved impossible to separate the effects of house age and construction methods. Although computed overland wave heights showed good correlation with fragility, comparison with the limited measured data available showed that wave heights were significantly overpredicted inland. Finally, more accurate standards for integrated and local loads on elevated houses will be necessary to perform detailed structural system-based analyses of fragility.

Coastal acidification in southeastern U.S. estuaries and coastal waters is influenced by biological activity, run-off from the land, and increasing carbon dioxide in the atmosphere. Acidification can negatively impact coastal resources such as shellfish, finfish, and coral reefs, and the communities that rely on them. Organismal responses for species located in the U.S. Southeast document large negative impacts of acidification, especially in larval stages. For example, the toxicity of pesticides increases under acidified conditions and the combination of acidification and low oxygen has profoundly negative influences on genes regulating oxygen consumption. In corals, the rate of calcification decreases with acidification and processes such as wound recovery, reproduction, and recruitment are negatively impacted. Minimizing the changes in global ocean chemistry will ultimately depend on the reduction of carbon dioxide emissions, but adaptation to these changes and mitigation of the local stressors that exacerbate global acidification can be addressed locally. The evolution of our knowledge of acidification, from basic understanding of the problem to the emergence of applied research and monitoring, has been facilitated by the development of regional Coastal Acidification Networks (CANs) across the United States. This synthesis is a product of the Southeast Coastal and Ocean Acidification Network (SOCAN). SOCAN was established to better understand acidification in the coastal waters of the U.S. Southeast and to foster communication among scientists, resource managers, businesses, and governments in the region. Here we review acidification issues in the U.S. Southeast, including the regional mechanisms of acidification and their potential impacts on biological resources and coastal communities. We recommend research and monitoring priorities and discuss the role SOCAN has in advancing acidification research and mitigation of and adaptation to these changes.

Fish represent the most diverse group of animals in the vertebrate phylum. The more than 25,000 species are characterized by an array of anatomical, biochemical, physiological, and behavioral repertoires. For this reason, it is difficult to develop a comprehensive guideline on the care and use of fishes. Institutional animal care and use committees (IACUCs) meet the challenge of ensuring adequate fish welfare using guidelines (Animal Welfare Act [AWA] and Public Health Service [PHS] Policy and their guides) derived mainly from the care and use of mammalian species, which may not be optimal for regulating fish research, teaching, or extension activities. Discussion focuses on various issues that often confront IACUCs in meeting regulatory requirements while assuring proper fish welfare. Issues include questions concerning animal tracking and inventory, utilization of fisheries bycatch, facility inspections in remote locations, and euthanasia. Common sense solutions appropriate for field and laboratory fish activities are suggested, which should help investigators, IACUCs, and regulatory agencies meet PHS and AWA objectives.

Abstract As states, cities, tribes, and private interests cope with climate damages and seek to increase preparedness and resilience, they will need to navigate myriad choices and options available to them. Making these choices in ways that identify pathways for climate action that support their development objectives will require constructive public dialogue, community participation, and flexible and ongoing access to science- and experience-based knowledge. In 2016, a Federal Advisory Committee (FAC) was convened to recommend how to conduct a sustained National Climate Assessment (NCA) to increase the relevance and usability of assessments for informing action. The FAC was disbanded in 2017, but members and additional experts reconvened to complete the report that is presented here. A key recommendation is establishing a new nonfederal “climate assessment consortium” to increase the role of state/local/tribal government and civil society in assessments. The expanded process would 1) focus on applied problems faced by practitioners, 2) organize sustained partnerships for collaborative learning across similar projects and case studies to identify effective tested practices, and 3) assess and improve knowledge-based methods for project implementation. Specific recommendations include evaluating climate models and data using user-defined metrics; improving benefit–cost assessment and supporting decision-making under uncertainty; and accelerating application of tools and methods such as citizen science, artificial intelligence, indicators, and geospatial analysis. The recommendations are the result of broad consultation and present an ambitious agenda for federal agencies, state/local/tribal jurisdictions, universities and the research sector, professional associations, nongovernmental and community-based organizations, and private-sector firms.

ABSTRACT Pond‐raised hybrid striped bass were commercially processed into fillets with and without belly flap, packaged in oxygen permeable bags (Cryovac E (tm) ), and stored as chillpack samples (‐2°C) and as refrigerated samples (2°C). Quality changes were determined over 21 days storage by microbiological, chemical, and sensory evaluations. Aerobic plate counts and hypoxanthine formation showed evidence of rapid deterioration of refrigerated samples after 8 days storage compared to 21 days for chillpack samples. A trained sensory panel found 21 day stored chillpack samples similar in flavor and aroma but of firmer texture than refrigerated samples stored 8 days. Panelists found no differences between trimmed and whole fillets. Hypoxanthine formation and aerobic plate counts appeared to be good indicators of quality deterioration, whereas thiobarbituric acid measurements were not.

A case-study approach was used to understand the role of social capital in the cycle of adaptive capacity in three rural, forest communities in Washington State. The study reveals social capital to be a critical ingredient in the resolution of diverse community development events. The findings enhance our understanding of the impacts of social capital on community outcomes by dividing the concept into three types—bonding, bridging, and linking social capital. Social capital does not translate linearly into community outcomes. Generally, community members stressed the importance of bridging social capital to achieve community-wide desired outcomes. Yet strong bridging social capital had no potency when linking social capital with key power brokers was absent. Finally, the case-study approach reveals how social capital is created and can be built up or depleted. The findings are applicable to community development practitioners, rural community leaders, and public land managers that interface with forest communities.

Abstract Aim Understanding the spatial ecology of animal movements is a critical element in conserving long‐lived, highly mobile marine species. Analyzing networks developed from movements of six sea turtle species reveals marine connectivity and can help prioritize conservation efforts. Location Global. Methods We collated telemetry data from 1235 individuals and reviewed the literature to determine our dataset's representativeness. We used the telemetry data to develop spatial networks at different scales to examine areas, connections, and their geographic arrangement. We used graph theory metrics to compare networks across regions and species and to identify the role of important areas and connections. Results Relevant literature and citations for data used in this study had very little overlap. Network analysis showed that sampling effort influenced network structure, and the arrangement of areas and connections for most networks was complex. However, important areas and connections identified by graph theory metrics can be different than areas of high data density. For the global network, marine regions in the Mediterranean had high closeness, while links with high betweenness among marine regions in the South Atlantic were critical for maintaining connectivity. Comparisons among species‐specific networks showed that functional connectivity was related to movement ecology, resulting in networks composed of different areas and links. Main conclusions Network analysis identified the structure and functional connectivity of the sea turtles in our sample at multiple scales. These network characteristics could help guide the coordination of management strategies for wide‐ranging animals throughout their geographic extent. Most networks had complex structures that can contribute to greater robustness but may be more difficult to manage changes when compared to simpler forms. Area‐based conservation measures would benefit sea turtle populations when directed toward areas with high closeness dominating network function. Promoting seascape connectivity of links with high betweenness would decrease network vulnerability.